RNA
Part:BBa_K4757031
Designed by: Marik Müller Group: iGEM23_Heidelberg (2023-10-06)
sRNA MicC targeting BBa_J61100
Composite part from BBa_K4757021 and BBa_K4757028. This composite can be used for sRNA mediated gene repression by targeting the RBS BBa_J61100. The part was tested by expressing the sRNA with the XylS/Pm expression cassette and targeting the RBS (BBa_J61100) in front of mKate2 (BBa_K4757000), expressed by the constitutive promoter pEM7 and measuring the decrease in fluorescens. The repression strength was calculated by dividing the mean fluorescens after induction with the fluorescens of the genetic construct lacking the sRNA gene. Measurements were performed in P. fluorescens.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 88
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |